› 7kay

X-ray diffraction
1.95Å resolution

Crystal structure of OhyA(E82A)-18:1 complex from Staphylococcus aureus

Released:
DOI: 10.2210/pdb7kay/pdb
PDB entry 7kay coloured by chain, front view.
PDB entry 7kay coloured by chain, side view.
PDB entry 7kay coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structure and mechanism of Staphylococcus aureus oleate hydratase (OhyA).
Radka CD, Batte JL, Frank MW, Young BM, Rock CO
OpenAccess logo J Biol Chem 296 100252
PMID: 33376139

Function and Biology Details

Reaction catalysed: 
(R)-10-hydroxystearate = oleate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-cross-reactive antigen Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 611 amino acids
Theoretical weight: 69.83 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0D6GJV1 (Residues: 1-591; Coverage: 100%)
Gene names: AC807_1707, CFN12_00295, EIG96_01565, EP54_06595, EQ90_12415, ERS092965_01887, ERS195417_02651, GO814_02765, GO942_14045, HT539_06305
Sequence domains: MCRA family

Ligands and Environments

3 bound ligands:
Ligand OLA 3 x OLA
Ligand GOL 2 x GOL
Ligand K 2 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 189.044Å b: 113.534Å c: 119.283Å
α: 90° β: 117.04° γ: 90°
R-values:
R R work R free
0.159 0.159 0.186
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Towards an understanding of oleate hydratases and their application in industrial processes.
Prem et al. (2022)
3 other articles

2 mentions without citation

Novel oleate hydratases and potential biotechnological applications.
Hagedoorn et al. (2021)
1 more