7kaz

X-ray diffraction
1.85Å resolution

Crystal structure of OhyA(E82A)-18:1/h18:0-FAD complex from Staphylococcus aureus

Released:
Model geometry
Fit model/data

Function and Biology Details

Reaction catalysed:
(R)-10-hydroxystearate = oleate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101138 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oleate hydratase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 611 amino acids
Theoretical weight: 69.83 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0D6GJV1 (Residues: 1-591; Coverage: 100%)
Gene names: ACR79_06910, CNH36_00305, EIH03_08870, EP54_06595, EQ90_12415, GF599_08435, GO814_02765, GO942_14045
Sequence domains: MCRA family

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
3 bound ligands:
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 188.022Å b: 113.085Å c: 118.393Å
α: 90° β: 116.91° γ: 90°
R-values:
R R work R free
0.194 0.194 0.249
Expression system: Escherichia coli