› 7kbc

X-ray diffraction
2.25Å resolution

The crystal structure of the 2009/H1N1/California PA endonuclease mutant E119D (construct with truncated loop 51-72) in complex with baloxavir acid

Released:
DOI: 10.2210/pdb7kbc/pdb
PDB entry 7kbc coloured by chain, front view.
PDB entry 7kbc coloured by chain, side view.
PDB entry 7kbc coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism.
Kumar G, Cuypers M, Webby RR, Webb TR, White SW
OpenAccess logo Nucleic Acids Res 49 1609-1618 (2021)
PMID: 33469660

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105425 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PA-X Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 23.13 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A5J6VBC3 (Residues: 1-50, 65-183; Coverage: 75%)
Gene name: PA-X
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA

Ligands and Environments

5 bound ligands:
Ligand E4Z 1 x E4Z
Ligand QQ4 1 x QQ4
Ligand GOL 5 x GOL
Ligand MN 2 x MN
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: I422
Unit cell:
a: 89.963Å b: 89.963Å c: 133.943Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Not making the cut: Techniques to prevent RNA cleavage in structural studies of RNase-RNA complexes.
Jones et al. (2022)
7 other articles

1 mention without citation

Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism.
Kumar et al. (2021)