7kbd

Electron Microscopy
3.38Å resolution

Nucleosome in interphase chromosome formed in Xenopus egg extract (oligo fraction)

Released:
Source organism: Xenopus laevis
Primary publication:
Structural features of nucleosomes in interphase and metaphase chromosomes.
Mol Cell 81 4377-4397.e12 (2021)
PMID: 34478647
Related structures: EMD-22790

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-121447 (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
Histone H3.2 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.42 KDa
Source organism: Xenopus laevis
UniProt:
  • Canonical: P84233 (Residues: 1-136; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A Chains: C, G
Molecule details ›
Chains: C, G
Length: 139 amino acids
Theoretical weight: 14.88 KDa
Source organism: Xenopus laevis
UniProt:
  • Canonical: Q6DKE3 (Residues: 1-139; Coverage: 100%)
Gene names: HIST1H2AH, LOC108709196, MGC81997, h2ac1, h2ac1.S, hist1h2aa, hist1h2aa.L
Sequence domains:
Histone H2B 1.1 Chains: D, H
Molecule details ›
Chains: D, H
Length: 126 amino acids
Theoretical weight: 13.97 KDa
Source organism: Xenopus laevis
UniProt:
  • Canonical: P02281 (Residues: 1-126; Coverage: 100%)
Sequence domains: Core histone H2A/H2B/H3/H4
DNA (151-MER) Chain: I
Molecule details ›
Chain: I
Length: 151 nucleotides
Theoretical weight: 46.85 KDa
DNA (151-MER) Chain: J
Molecule details ›
Chain: J
Length: 151 nucleotides
Theoretical weight: 46.36 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.38Å
Relevant EMDB volumes: EMD-22790