› 7ke3

X-ray diffraction
2.2Å resolution

Heavy chain ferritin with C-terminal EBNA1 epitope

Released:
DOI: 10.2210/pdb7ke3/pdb
PDB entry 7ke3 coloured by chain, front view.
PDB entry 7ke3 coloured by chain, side view.
PDB entry 7ke3 coloured by chain, top view.
Source organisms:
Primary publication:
Immunogenicity study of engineered ferritins with C- and N-terminus insertion of Epstein-Barr nuclear antigen 1 epitope.
Qu Y, Zhang B, Wang Y, Yin S, Pederick JL, Bruning JB, Sun Y, Middelberg A, Bi J
Vaccine 39 4830-4841 (2021)
PMID: 34284876

Function and Biology Details

Reaction catalysed: 
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136240 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Epstein-Barr nuclear antigen 1; Ferritin heavy chain Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 209 amino acids
Theoretical weight: 23.51 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P02794 (Residues: 1-183; Coverage: 100%)
  • Canonical: P03211 (Residues: 407-417; Coverage: 2%)
Gene names: BKRF1, EBNA1, FTH, FTH1, FTHL6, OK/SW-cl.84, PIG15
Sequence domains: Ferritin-like domain

Ligands and Environments

2 bound ligands:
Ligand TRS 6 x TRS
Ligand FE 20 x FE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P42212
Unit cell:
a: 217.882Å b: 217.882Å c: 147.203Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Biological Nanoparticles in Vaccine Development.
Curley et al. (2022)
1 more