› 7ken

X-ray diffraction
1.8Å resolution

Protein Tyrosine Phosphatase 1B, D289A mutant, apo state

Released:
DOI: 10.2210/pdb7ken/pdb
PDB entry 7ken coloured by chain, front view.
PDB entry 7ken coloured by chain, side view.
PDB entry 7ken coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Optogenetic Analysis of Allosteric Control in Protein Tyrosine Phosphatases.
Hongdusit A, Fox JM
Biochemistry 60 254-258 (2021)
PMID: 33450156

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 329 amino acids
Theoretical weight: 38.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18031 (Residues: 2-323; Coverage: 74%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P3121
Unit cell:
a: 88.314Å b: 88.314Å c: 104.155Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.244
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Optogenetics in bacteria - applications and opportunities.
Lindner et al. (2022)
1 more

1 mention without citation

Native dynamics and allosteric responses in PTP1B probed by high-resolution HDX-MS.
Woods et al. (2023)