› 7kex

Electron Microscopy
4.25Å resolution

Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-293

Released:
DOI: 10.2210/pdb7kex/pdb
PDB entry 7kex coloured by chain, front view.
PDB entry 7kex coloured by chain, side view.
PDB entry 7kex coloured by chain, top view.
Source organisms:
Primary publication:
Convergence of a common solution for broad ebolavirus neutralization by glycan cap-directed human antibodies.
Murin CD, Gilchuk P, Ilinykh PA, Huang K, Kuzmina N, Shen X, Bruhn JF, Bryan AL, Davidson E, Doranz BJ, Williamson LE, Copps J, Alkutkar T, Flyak AI, Bukreyev A, Crowe JE, Ward AB
OpenAccess logo Cell Rep 35 108984 (2021)
PMID: 33852862
Related structures: EMD-22842

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211931 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Antibody Fab EBOV-293 light chain Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 232 amino acids
Theoretical weight: 25.22 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Antibody Fab EBOV-293 heavy chain Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 244 amino acids
Theoretical weight: 26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
GP1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 313 amino acids
Theoretical weight: 34.98 KDa
Source organism: Ebola virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 32-309; Coverage: 43%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
GP2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 203 amino acids
Theoretical weight: 22.16 KDa
Source organism: Ebola virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 461-629; Coverage: 26%)
Gene name: GP
Sequence domains: Envelope glycoprotein GP2-like, HR1-HR2

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.25Å
Relevant EMDB volumes: EMD-22842
Expression system: Homo sapiens

Quick links

Citations

4 review citations

Filovirus Neutralising Antibodies: Mechanisms of Action and Therapeutic Application.
Hargreaves et al. (2021)
3 more

2 mentions without citation

Convergence of a common solution for broad ebolavirus neutralization by glycan cap-directed human antibodies.
Murin et al. (2021)
1 more