7kgj

X-ray diffraction
2.3Å resolution

Crystal structure of synthetic nanobody (Sb45) complexes with SARS-CoV-2 receptor binding domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-534814 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spike protein S1 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0DTC2 (Residues: 333-528; Coverage: 16%)
Gene names: 2, S
Sequence domains: Betacoronavirus spike glycoprotein S1, receptor binding
Sb45, Sybody-45, Synthetic Nanobody Chain: B
Molecule details ›
Chain: B
Length: 121 amino acids
Theoretical weight: 13.57 KDa
Source organism: synthetic construct
Expression system: Escherichia coli MC1061

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3221
Unit cell:
a: 62.552Å b: 62.552Å c: 168.815Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.186 0.216
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli MC1061