› 7kig

X-ray diffraction
1.4Å resolution

Crystal structure of the MarR family transcriptional regulator from Variovorax paradoxus bound to Indole-3-butyric acid

Released:
DOI: 10.2210/pdb7kig/pdb
PDB entry 7kig coloured by chain, front view.
PDB entry 7kig coloured by chain, side view.
PDB entry 7kig coloured by chain, top view.
Source organism: Variovorax paradoxus
Primary publication:
Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome.
Conway JM, Walton WG, Salas-González I, Law TF, Lindberg CA, Crook LE, Kosina SM, Fitzpatrick CR, Lietzan AD, Northen TR, Jones CD, Finkel OM, Redinbo MR, Dangl JL
OpenAccess logo Nat Microbiol 7 1817-1833 (2022)
PMID: 36266335

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH marR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 20.97 KDa
Source organism: Variovorax paradoxus
Expression system: Escherichia coli
UniProt:
  • Canonical: C5CSP2 (Residues: 1-158; Coverage: 100%)
Gene name: Vapar_1489
Sequence domains: MarR family

Ligands and Environments

2 bound ligands:
Ligand 3IB 1 x 3IB
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: F222
Unit cell:
a: 55.007Å b: 114.232Å c: 116.595Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.2
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The birth of a giant: evolutionary insights into the origin of auxin responses in plants.
Carrillo-Carrasco et al. (2023)
3 more