› 7kjy

Electron Microscopy
3.2Å resolution

Symmetry in Yeast Alcohol Dehydrogenase 1 - Open Form with NADH

Released:
DOI: 10.2210/pdb7kjy/pdb
PDB entry 7kjy coloured by chain, front view.
PDB entry 7kjy coloured by chain, side view.
PDB entry 7kjy coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cryo-Electron Microscopy Structures of Yeast Alcohol Dehydrogenase.
Guntupalli SR, Li Z, Chang L, Plapp BV, Subramanian R
Biochemistry 60 663-677 (2021)
PMID: 33620215
Related structures: EMD-22902

Function and Biology Details

Reaction catalysed: 
A primary alcohol + NAD(+) = an aldehyde + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-579459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alcohol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 348 amino acids
Theoretical weight: 36.88 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: S5RZC2 (Residues: 1-348; Coverage: 100%)
Gene names: ADH1, YOL086C
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
1 bound ligand:
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-22902

Quick links

Citations

1 review citation

The metal cofactor: stationary or mobile?
Hagedoorn et al. (2024)
10 other articles

1 mention without citation

The Thr45Gly substitution in yeast alcohol dehydrogenase substantially decreases catalysis, alters pH dependencies, and disrupts the proton relay system.
Pal et al. (2021)