› 7kmf

Electron Microscopy
2.91Å resolution

Sugar phosphate activation of the stress sensor eIF2B

Released:
DOI: 10.2210/pdb7kmf/pdb
PDB entry 7kmf coloured by chain, front view.
PDB entry 7kmf coloured by chain, side view.
PDB entry 7kmf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Sugar phosphate activation of the stress sensor eIF2B.
Hao Q, Heo JM, Nocek BP, Hicks KG, Stoll VS, Remarcik C, Hackett S, LeBon L, Jain R, Eaton D, Rutter J, Wong YL, Sidrauski C
OpenAccess logo Nat Commun 12 3440 (2021)
PMID: 34103529
Related structures: EMD-22924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156021 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 367 amino acids
Theoretical weight: 41.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49770 (Residues: 1-351; Coverage: 100%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit delta Chains: E, F
Molecule details ›
Chains: E, F
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit alpha Chains: G, H
Molecule details ›
Chains: G, H
Length: 377 amino acids
Theoretical weight: 40.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit epsilon Chains: B, I
Molecule details ›
Chains: B, I
Length: 721 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon
Translation initiation factor eIF2B subunit gamma Chains: J, K
Molecule details ›
Chains: J, K
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase

Ligands and Environments

1 bound ligand:
Ligand F6P 2 x F6P
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.91Å
Relevant EMDB volumes: EMD-22924
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Surviving and Adapting to Stress: Translational Control and the Integrated Stress Response.
Wek et al. (2023)
3 more

1 mention without citation

Sugar phosphate activation of the stress sensor eIF2B.
Hao et al. (2021)