› 7koo

Electron Microscopy
3Å resolution

Alpha-7 nicotinic acetylcholine receptor bound to alpha-bungarotoxin in a resting state

Released:
DOI: 10.2210/pdb7koo/pdb
PDB entry 7koo coloured by chain, front view.
PDB entry 7koo coloured by chain, side view.
PDB entry 7koo coloured by chain, top view.
Source organisms:
Primary publication:
Structure and gating mechanism of the α7 nicotinic acetylcholine receptor.
Noviello CM, Gharpure A, Mukhtasimova N, Cabuco R, Baxter L, Borek D, Sine SM, Hibbs RE
Cell 184 2121-2134.e13 (2021)
PMID: 33735609
Related structures: EMD-22979

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142080 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Neuronal acetylcholine receptor subunit alpha-7; Soluble cytochrome b562 Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 562 amino acids
Theoretical weight: 63.83 KDa
Source organisms: Expression system: Homo sapiens
UniProt:
  • Canonical: P36544 (Residues: 24-371, 407-502; Coverage: 93%)
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
Gene names: CHRNA7, NACHRA7, cybC
Sequence domains:
Alpha-bungarotoxin isoform V31 Chains: F, G, H, I, J
Molecule details ›
Chains: F, G, H, I, J
Length: 71 amino acids
Theoretical weight: 7.72 KDa
Source organism: Bungarus multicinctus
Expression system: Not provided
UniProt:
  • Canonical: P60616 (Residues: 22-92; Coverage: 96%)
Sequence domains: Snake toxin cobra-type

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 5 x NAG
Ligand CA 5 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3Å
Relevant EMDB volumes: EMD-22979
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

12 review citations

Nicotinic acetylcholine receptors: Therapeutic targets for novel ligands to treat pain and inflammation.
Hone et al. (2023)
11 more

17 mentions without citation

Pursuing High-Resolution Structures of Nicotinic Acetylcholine Receptors: Lessons Learned from Five Decades.
Delgado-Vélez et al. (2021)
16 more