› 7kpr

X-ray diffraction
3.09Å resolution

Structure of wild-type PPM1H phosphatase at 3.1 Angstrom resolution

Released:
DOI: 10.2210/pdb7kpr/pdb
PDB entry 7kpr coloured by chain, front view.
PDB entry 7kpr coloured by chain, side view.
PDB entry 7kpr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the specificity of PPM1H phosphatase for Rab GTPases.
Waschbüsch D, Berndsen K, Lis P, Knebel A, Lam YP, Alessi DR, Khan AR
OpenAccess logo EMBO Rep 22 e52675 (2021)
PMID: 34580980

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein phosphatase 1H Chains: A, B
Molecule details ›
Chains: A, B
Length: 486 amino acids
Theoretical weight: 53.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULR3 (Residues: 33-514; Coverage: 94%)
Gene names: ARHCL1, KIAA1157, PPM1H, URCC2
Sequence domains: Protein phosphatase 2C

Ligands and Environments

1 bound ligand:
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: P212121
Unit cell:
a: 69.684Å b: 102.16Å c: 148.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.22
Expression system: Escherichia coli

Quick links

Citations

1 review citation

LRRK2 at Striatal Synapses: Cell-Type Specificity and Mechanistic Insights.
Skelton et al. (2022)
2 other articles

1 mention without citation

Structural basis for the specificity of PPM1H phosphatase for Rab GTPases.
Waschbüsch et al. (2021)