› 7kqx

X-ray diffraction
1.6Å resolution

MIF Y99C homotrimeric mutant

Released:
DOI: 10.2210/pdb7kqx/pdb
Model geometry
Fit model/data
PDB entry 7kqx coloured by chain, front view.
PDB entry 7kqx coloured by chain, side view.
PDB entry 7kqx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies.
Skeens E, Pantouris G, Shah D, Manjula R, Ombrello MJ, Maluf NK, Bhandari V, Lisi GP, Lolis EJ
OpenAccess logo Front Mol Biosci 9 783669 (2022)
PMID: 35252348

Function and Biology Details

Reactions catalysed: 
Keto-phenylpyruvate = enol-phenylpyruvate
L-dopachrome = 5,6-dihydroxyindole-2-carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146863 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage migration inhibitory factor Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 114 amino acids
Theoretical weight: 12.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14174 (Residues: 2-115; Coverage: 99%)
Gene names: GLIF, MIF, MMIF
Sequence domains: Macrophage migration inhibitory factor (MIF)

Ligands and Environments

3 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 15 x GOL
Ligand IPA 5 x IPA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 67.984Å b: 68.062Å c: 86.737Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.18
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

A Cysteine Variant at an Allosteric Site Alters MIF Dynamics and Biological Function in Homo- and Heterotrimeric Assemblies.
Skeens et al. (2022)