› 7ksq

Electron Microscopy
2.8Å resolution

The Structure of the moss PSI-LHCI reveals the evolution of the LHCI antenna

Released:
DOI: 10.2210/pdb7ksq/pdb
PDB entry 7ksq coloured by chain, front view.
PDB entry 7ksq coloured by chain, side view.
PDB entry 7ksq coloured by chain, top view.
Source organism: Physcomitrium patens
Primary publication:
The structure of the Physcomitrium patens photosystem I reveals a unique Lhca2 paralogue replacing Lhca4.
Gorski C, Riddle R, Toporik H, Da Z, Dobson Z, Williams D, Mazor Y
Nat Plants 8 307-316 (2022)
PMID: 35190662
Related structures: EMD-23023

Function and Biology Details

Reaction catalysed: 
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octadecamer (preferred)
PDBe Complex ID:
PDB-CPX-104274 (preferred)
Entry contents:
18 distinct polypeptide molecules
Macromolecules (18 distinct):
Photosystem I P700 chlorophyll a apoprotein A1 Chain: A
Molecule details ›
Chain: A
Length: 742 amino acids
Theoretical weight: 82.31 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: Q8MFA3 (Residues: 9-750; Coverage: 99%)
Gene name: psaA
Sequence domains: Photosystem I psaA/psaB protein
Photosystem I P700 chlorophyll a apoprotein A2 Chain: B
Molecule details ›
Chain: B
Length: 732 amino acids
Theoretical weight: 82.25 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: Q8MFA2 (Residues: 3-734; Coverage: 100%)
Gene name: psaB
Sequence domains: Photosystem I psaA/psaB protein
Chlorophyll a-b binding protein, chloroplastic Chain: 1
Molecule details ›
Chain: 1
Length: 192 amino acids
Theoretical weight: 20.76 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1JLZ3 (Residues: 50-241; Coverage: 78%)
Gene name: PHYPA_017397
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 2
Molecule details ›
Chain: 2
Length: 203 amino acids
Theoretical weight: 22.12 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1IW94 (Residues: 65-267; Coverage: 74%)
Gene name: PHYPA_025495
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 3
Molecule details ›
Chain: 3
Length: 218 amino acids
Theoretical weight: 23.83 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1IB10 (Residues: 103-320; Coverage: 68%)
Gene name: PHYPA_031029
Sequence domains: Chlorophyll A-B binding protein
Chlorophyll a-b binding protein, chloroplastic Chain: 4
Molecule details ›
Chain: 4
Length: 203 amino acids
Theoretical weight: 22.31 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1K0E4 (Residues: 65-267; Coverage: 75%)
Gene name: PHYPA_014361
Sequence domains: Chlorophyll A-B binding protein
Photosystem I iron-sulfur center Chain: C
Molecule details ›
Chain: C
Length: 80 amino acids
Theoretical weight: 8.65 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: Q6YXQ2 (Residues: 2-81; Coverage: 99%)
Gene name: psaC
Sequence domains: 4Fe-4S dicluster domain
Photosystem I reaction center subunit II, chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 142 amino acids
Theoretical weight: 15.79 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A9REG3 (Residues: 69-210; Coverage: 68%)
Gene name: PHYPA_021401
Sequence domains: PsaD
PsaE Chain: E
Molecule details ›
Chain: E
Length: 63 amino acids
Theoretical weight: 7.08 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1IHL7 (Residues: 69-131; Coverage: 48%)
Gene name: PHYPA_029370
Sequence domains: Photosystem I reaction centre subunit IV / PsaE
Photosystem I reaction center subunit III Chain: F
Molecule details ›
Chain: F
Length: 160 amino acids
Theoretical weight: 17.65 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1IN36 (Residues: 87-246; Coverage: 65%)
Gene name: PHYPA_026994
Sequence domains: Photosystem I reaction centre subunit III
Photosystem I reaction center subunit V, chloroplastic Chain: G
Molecule details ›
Chain: G
Length: 91 amino acids
Theoretical weight: 9.73 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1JC42 (Residues: 59-149; Coverage: 59%)
Gene name: PHYPA_019380
Sequence domains: Photosystem I psaG / psaK
Photosystem I reaction center subunit VI, chloroplastic Chain: H
Molecule details ›
Chain: H
Length: 87 amino acids
Theoretical weight: 9.49 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1JDR4 (Residues: 52-138; Coverage: 63%)
Gene name: PHYPA_019944
Sequence domains: Photosystem I reaction centre subunit VI
Photosystem I reaction center subunit VIII Chain: I
Molecule details ›
Chain: I
Length: 34 amino acids
Theoretical weight: 3.79 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: Q6YXR3 (Residues: 1-34; Coverage: 94%)
Gene names: Pp3P_310V3, psaI
Sequence domains: Photosystem I reaction centre subunit VIII
Photosystem I reaction center subunit IX Chain: J
Molecule details ›
Chain: J
Length: 41 amino acids
Theoretical weight: 4.6 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: Q6YXM2 (Residues: 1-41; Coverage: 100%)
Gene name: psaJ
Sequence domains: Photosystem I reaction centre subunit IX / PsaJ
PSI-K Chain: K
Molecule details ›
Chain: K
Length: 81 amino acids
Theoretical weight: 8.11 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1JLW2 (Residues: 49-129; Coverage: 62%)
Gene name: PHYPA_017195
Sequence domains: Photosystem I psaG / psaK
PSI subunit V Chain: L
Molecule details ›
Chain: L
Length: 160 amino acids
Theoretical weight: 16.69 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1KIF2 (Residues: 66-225; Coverage: 71%)
Gene name: PHYPA_007223
Sequence domains: Photosystem I reaction centre subunit XI
Photosystem I reaction center subunit XII Chain: M
Molecule details ›
Chain: M
Length: 30 amino acids
Theoretical weight: 3.18 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: Q6YXK4 (Residues: 3-32; Coverage: 94%)
Gene names: Pp3P_710V3, psaM
Sequence domains: Photosystem I protein M (PsaM)
Photosystem I subunit O Chain: O
Molecule details ›
Chain: O
Length: 88 amino acids
Theoretical weight: 9.78 KDa
Source organism: Physcomitrium patens
UniProt:
  • Canonical: A0A2K1JDE1 (Residues: 56-143; Coverage: 61%)
Gene name: PHYPA_019829
Sequence domains: PsaO transmembrane domain

Ligands and Environments

11 bound ligands:
Ligand CL0 1 x CL0
Ligand CLA 144 x CLA
Ligand PQN 2 x PQN
Ligand SF4 3 x SF4
Ligand BCR 27 x BCR
Ligand LHG 7 x LHG
Ligand LMG 4 x LMG
Ligand LMT 6 x LMT
Ligand DGD 1 x DGD
Ligand CHL 14 x CHL
Ligand LUT 10 x LUT
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.8Å
Relevant EMDB volumes: EMD-23023

Quick links

Citations

5 citation in other articles

Growth and Photosynthetic Efficiency of Microalgae and Plants with Different Levels of Complexity Exposed to a Simulated M-Dwarf Starlight.
Battistuzzi et al. (2023)
4 more

1 mention without citation

Dynamic Regulation of the Light-Harvesting System through State Transitions in Land Plants and Green Algae.
Shang et al. (2023)