7ktp

Electron Microscopy
4.8Å resolution

PRC2:EZH1_B from a dimeric PRC2 bound to a nucleosome

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-131028 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Histone-lysine N-methyltransferase EZH1 Chain: A
Molecule details ›
Chain: A
Length: 747 amino acids
Theoretical weight: 85.39 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q92800 (Residues: 1-747; Coverage: 100%)
Gene names: EZH1, KIAA0388
Sequence domains:
Polycomb protein EED Chain: B
Molecule details ›
Chain: B
Length: 441 amino acids
Theoretical weight: 50.27 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75530 (Residues: 1-441; Coverage: 100%)
Gene name: EED
Sequence domains: WD domain, G-beta repeat
Polycomb protein SUZ12 Chain: C
Molecule details ›
Chain: C
Length: 739 amino acids
Theoretical weight: 83.18 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15022 (Residues: 1-739; Coverage: 100%)
Gene names: CHET9, JJAZ1, KIAA0160, SUZ12
Sequence domains: VEFS-Box of polycomb protein
Histone-binding protein RBBP4 Chain: D
Molecule details ›
Chain: D
Length: 425 amino acids
Theoretical weight: 47.71 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q09028 (Residues: 1-425; Coverage: 100%)
Gene names: RBAP48, RBBP4
Sequence domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-23025
Expression system: Spodoptera frugiperda