7ky6

Electron Microscopy
3.1Å resolution

Structure of the S. cerevisiae phosphatidylcholine flippase Dnf1-Lem3 complex in the apo E1 state

Released:
Related structures: EMD-23069

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-247687 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Phospholipid-transporting ATPase DNF1 Chain: A
Molecule details ›
Chain: A
Length: 1571 amino acids
Theoretical weight: 178 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
  • Canonical: P32660 (Residues: 1-1571; Coverage: 100%)
Gene names: DNF1, SYGP-ORF7, YER166W
Sequence domains:
Alkylphosphocholine resistance protein LEM3 Chain: B
Molecule details ›
Chain: B
Length: 414 amino acids
Theoretical weight: 47.49 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-23069
Expression system: Saccharomyces cerevisiae S288C