› 7kz8

X-ray diffraction
2.2Å resolution

Crystal structure of substrate-binding protein Aapf from Pseudomonas sp. PDC86

Released:
DOI: 10.2210/pdb7kz8/pdb
PDB entry 7kz8 coloured by chain, front view.
PDB entry 7kz8 coloured by chain, side view.
PDB entry 7kz8 coloured by chain, top view.
Source organism: Pseudomonas sp. PDC86
Primary publication:
Structural basis for a bacterial Pip system plant effector recognition protein.
Luo S, Coutinho BG, Dadhwal P, Oda Y, Ren J, Schaefer AL, Greenberg EP, Harwood CS, Tong L
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33649224

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptide/nickel transport system substrate-binding protein AapF Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 55.09 KDa
Source organism: Pseudomonas sp. PDC86
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P21
Unit cell:
a: 62.252Å b: 41.961Å c: 93.143Å
α: 90° β: 99.55° γ: 90°
R-values:
R R work R free
0.22 0.218 0.267
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cell-Cell Signaling Proteobacterial LuxR Solos: a Treasure Trove of Subgroups Having Different Origins, Ligands, and Ecological Roles.
Bez et al. (2023)
1 more