7kzt

Electron Microscopy
4.2Å resolution

Structure of the human fanconi anaemia Core-UBE2T-ID-DNA complex in intermediate state

Released:
Primary publication:
Structure of the FA core ubiquitin ligase closing the ID clamp on DNA.
Nat Struct Mol Biol 28 300-309 (2021)
PMID: 33686268
Related structures: EMD-23089

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero nonadecamer (preferred)
PDBe Complex ID:
PDB-CPX-110659 (preferred)
Entry contents:
12 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (14 distinct):
Fanconi anemia group A protein Chains: A, S
Molecule details ›
Chains: A, S
Length: 1477 amino acids
Theoretical weight: 165.51 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fanconi anemia group B protein Chains: B, O
Molecule details ›
Chains: B, O
Length: 884 amino acids
Theoretical weight: 100.64 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Fanconi anemia group C protein Chain: C
Fanconi anemia group E protein Chain: E
Fanconi anemia group F protein Chain: F
Fanconi anemia group G protein Chains: G, H
E3 ubiquitin-protein ligase FANCL Chains: L, M
Fanconi anemia core complex-associated protein 100 Chains: P, Q
Fanconi anemia core complex-associated protein 20 Chain: W
Ubiquitin-conjugating enzyme E2 T Chain: X
Fanconi anemia, complementation group I Chain: U
Fanconi anemia group D2 protein Chain: V
DNA (22-MER) Chain: Y
DNA (22-MER) Chain: Z

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-23089
Expression systems:
  • Homo sapiens
  • Escherichia coli
  • Spodoptera frugiperda
  • Not provided