› 7kzx

Electron Microscopy
4Å resolution

Cryo-EM structure of YiiP-Fab complex in Apo state

Released:
DOI: 10.2210/pdb7kzx/pdb
PDB entry 7kzx coloured by chain, front view.
PDB entry 7kzx coloured by chain, side view.
PDB entry 7kzx coloured by chain, top view.
Source organisms:
Primary publication:
Zinc binding alters the conformational dynamics and drives the transport cycle of the cation diffusion facilitator YiiP.
Lopez-Redondo M, Fan S, Koide A, Koide S, Beckstein O, Stokes DL
OpenAccess logo J Gen Physiol 153 (2021)
PMID: 34254979
Related structures: EMD-23092

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212570 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cation-efflux pump FieF Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 32.49 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli
Fab2R light chain Chains: C, E
Molecule details ›
Chains: C, E
Length: 216 amino acids
Theoretical weight: 23.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab2R heavy chain Chains: D, F
Molecule details ›
Chains: D, F
Length: 238 amino acids
Theoretical weight: 25.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-23092
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Flipping the switch: dynamic modulation of membrane transporter activity in bacteria.
Elston et al. (2023)
1 more

6 mentions without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)
5 more