› 7l0i

X-ray diffraction
2.02Å resolution

Ligand-free YopH G352T

Released:
DOI: 10.2210/pdb7l0i/pdb
PDB entry 7l0i coloured by chain, front view.
PDB entry 7l0i coloured by chain, side view.
PDB entry 7l0i coloured by chain, top view.
Source organism: Yersinia pestis
Primary publication:
Single Residue on the WPD-Loop Affects the pH Dependency of Catalysis in Protein Tyrosine Phosphatases.
Shen R, Crean RM, Johnson SJ, Kamerlin SCL, Hengge AC
OpenAccess logo JACS Au 1 646-659 (2021)
PMID: 34308419

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein-tyrosine-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.6 KDa
Source organism: Yersinia pestis
Expression system: Escherichia coli
UniProt:
  • Canonical: O68720 (Residues: 164-468; Coverage: 65%)
Gene names: Y0013, YPCD1.67c, yopH
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

1 bound ligand:
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 49.135Å b: 56.296Å c: 98.335Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.253
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)
2 other articles