7l1v

Electron Microscopy
3Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-520123 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Engineered Guanine nucleotide-binding protein subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 244 amino acids
Theoretical weight: 28.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 349 amino acids
Theoretical weight: 38.58 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: C
Molecule details ›
Chain: C
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
single-chain antibody Fv fragment (svFv16) Chain: H
Molecule details ›
Chain: H
Length: 250 amino acids
Theoretical weight: 26.61 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Orexin receptor type 2 Chain: R
Molecule details ›
Chain: R
Length: 374 amino acids
Theoretical weight: 43.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: O43614 (Residues: 3-389; Coverage: 80%)
Gene name: HCRTR2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Synthetic nanobody 51 (Sb51) Chain: S
Molecule details ›
Chain: S
Length: 118 amino acids
Theoretical weight: 12.79 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-23119
Expression systems:
  • Escherichia coli
  • Trichoplusia ni
  • Homo sapiens