› 7l7e

X-ray diffraction
3Å resolution

Crystal structure of SARS-CoV-2 spike RBD in complex with human monoclonal antibodies AZD8895 and AZD1061

Released:
DOI: 10.2210/pdb7l7e/pdb
Model geometry
Fit model/data
PDB entry 7l7e coloured by chain, front view.
PDB entry 7l7e coloured by chain, side view.
PDB entry 7l7e coloured by chain, top view.
Source organisms:
Primary publication:
Genetic and structural basis for SARS-CoV-2 variant neutralization by a two-antibody cocktail.
Dong J, Zost SJ, Greaney AJ, Starr TN, Dingens AS, Chen EC, Chen RE, Case JB, Sutton RE, Gilchuk P, Rodriguez J, Armstrong E, Gainza C, Nargi RS, Binshtein E, Xie X, Zhang X, Shi PY, Logue J, Weston S, McGrath ME, Frieman MB, Brady T, Tuffy KM, Bright H, Loo YM, McTamney PM, Esser MT, Carnahan RH, Diamond MS, Bloom JD, Crowe JE
OpenAccess logo Nat Microbiol 6 1233-1244 (2021)
PMID: 34548634

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-209813 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
heavy chain of human monoclonal antibody AZD8895 Chains: A, H, I, T
Molecule details ›
Chains: A, H, I, T
Length: 225 amino acids
Theoretical weight: 23.94 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
light chain of human monoclonal antibody Fab AZD8895 Chains: B, J, L, U
Molecule details ›
Chains: B, J, L, U
Length: 216 amino acids
Theoretical weight: 23.57 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
heavy chain of human monoclonal antibody Fab AZD1061 Chains: C, E, M, O
light chain of human monoclonal antibody Fab AZD1061 Chains: D, F, N, P
Spike protein S1 Chains: G, K, S, b

Ligands and Environments

2 bound ligands:
Ligand PO4 8 x PO4
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 97.152Å b: 152.525Å c: 199.244Å
α: 90° β: 94.74° γ: 90°
R-values:
R R work R free
0.218 0.215 0.273
Expression system: Homo sapiens

Quick links

Citations

41 review citations

Tackling COVID-19 with neutralizing monoclonal antibodies.
Corti et al. (2021)
40 more

48 mentions without citation

Monoclonal antibody therapies against SARS-CoV-2.
Focosi et al. (2022)
47 more