› 7l8v

Solution NMR

NMR Structure of half-calcified calmodulin mutant (CaMEF12) bound to the IQ-motif of CaV1.2

Released:
DOI: 10.2210/pdb7l8v/pdb
PDB entry 7l8v coloured by chain, ensemble of 12 models, front view.
PDB entry 7l8v coloured by chain, ensemble of 12 models, side view.
PDB entry 7l8v coloured by chain, ensemble of 12 models, top view.
Source organism: Homo sapiens
Primary publication:
Half-calcified calmodulin promotes basal activity and inactivation of the L-type calcium channel CaV1.2.
Bartels P, Salveson I, Coleman AM, Anderson DE, Jeng G, Estrada-Tobar ZM, Man KNM, Yu Q, Kuzmenkina E, Nieves-Cintron M, Navedo MF, Horne MC, Hell JW, Ames JB
OpenAccess logo J Biol Chem 298 102701 (2022)
PMID: 36395884

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143947 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Voltage-dependent L-type calcium channel subunit alpha-1C Chain: C
Molecule details ›
Chain: C
Length: 21 amino acids
Theoretical weight: 2.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13936 (Residues: 1662-1682; Coverage: 1%)
Gene names: CACH2, CACN2, CACNA1C, CACNL1A1, CCHL1A1

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 49%
Refinement method: DGSA-distance geometry simulated annealing
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Regulation of Cardiac Cav1.2 Channels by Calmodulin.
Kameyama et al. (2023)
2 more

1 mention without citation

Half-calcified calmodulin promotes basal activity and inactivation of the L-type calcium channel CaV1.2.
Bartels et al. (2022)