7lbm

Electron Microscopy
4.8Å resolution

Structure of the human Mediator-bound transcription pre-initiation complex

Released:
Source organism: Homo sapiens
Related structures: EMD-23255

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
ATP + H(2)O = ADP + phosphate
ATP + a protein = ADP + a phosphoprotein
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 56-mer (preferred)
PDBe Complex ID:
PDB-CPX-106077 (preferred)
Entry contents:
54 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (56 distinct):
Mediator of RNA polymerase II transcription subunit 16 Chain: 0
Molecule details ›
Chain: 0
Length: 841 amino acids
Theoretical weight: 93.14 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9Y2X0 (Residues: 1-828; Coverage: 94%)
  • Best match: Q9Y2X0-2 (Residues: 1-841)
Gene names: DRIP92, MED16, THRAP5
Sequence domains:
Mediator of RNA polymerase II transcription subunit 23 Chain: 1
Molecule details ›
Chain: 1
Length: 1368 amino acids
Theoretical weight: 156.65 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9ULK4 (Residues: 1-1368; Coverage: 100%)
Gene names: ARC130, CRSP3, DRIP130, KIAA1216, MED23, SUR2
Sequence domains: Mediator complex subunit 23
Mediator of RNA polymerase II transcription subunit 24 Chain: 2
Mediator of RNA polymerase II transcription subunit 25 Chain: 3
DNA-directed RNA polymerase II subunit RPB1 Chain: A
DNA-directed RNA polymerase II subunit RPB2 Chain: B
DNA-directed RNA polymerase II subunit RPB3 Chain: C
DNA-directed RNA polymerase II subunit RPB4 Chain: D
DNA-directed RNA polymerases I, II, and III subunit RPABC1 Chain: E
DNA-directed RNA polymerases I, II, and III subunit RPABC2 Chain: F
DNA-directed RNA polymerase II subunit RPB7 Chain: G
DNA-directed RNA polymerases I, II, and III subunit RPABC3 Chain: H
DNA-directed RNA polymerase II subunit RPB9 Chain: I
DNA-directed RNA polymerases I, II, and III subunit RPABC5 Chain: J
DNA-directed RNA polymerase II subunit RPB11-a Chain: K
DNA-directed RNA polymerases I, II, and III subunit RPABC4 Chain: L
Transcription initiation factor IIA subunit 1 Chain: M
Transcription initiation factor IIA subunit 2 Chain: N
Transcription initiation factor IIB Chain: O
TATA-box-binding protein Chain: P
General transcription factor IIE subunit 1 Chain: Q
Transcription initiation factor IIE subunit beta Chain: R
General transcription factor IIF subunit 1 Chain: S
General transcription factor IIF subunit 2 Chain: T
General transcription and DNA repair factor IIH helicase/translocase subunit XPB Chain: W
General transcription and DNA repair factor IIH helicase subunit XPD Chain: X
General transcription factor IIH subunit 1 Chain: Y
General transcription factor IIH subunit 4 Chain: Z
General transcription factor IIH subunit 2 Chain: a
General transcription factor IIH subunit 3 Chain: b
General transcription factor IIH subunit 5 Chain: c
CDK-activating kinase assembly factor MAT1 Chain: d
Cyclin-dependent kinase 7 Chain: e
Cyclin-H Chain: f
Mediator of RNA polymerase II transcription subunit 6 Chain: g
Mediator of RNA polymerase II transcription subunit 8 Chain: h
Mediator of RNA polymerase II transcription subunit 11 Chain: i
Mediator of RNA polymerase II transcription subunit 17 Chain: j
Mediator of RNA polymerase II transcription subunit 18 Chain: k
Mediator of RNA polymerase II transcription subunit 20 Chain: l
Mediator of RNA polymerase II transcription subunit 22 Chain: m
Mediator of RNA polymerase II transcription subunit 27 Chain: n
Mediator of RNA polymerase II transcription subunit 28 Chain: o
Mediator of RNA polymerase II transcription subunit 29 Chain: p
Mediator of RNA polymerase II transcription subunit 30 Chain: q
Mediator of RNA polymerase II transcription subunit 14 Chain: r
Mediator of RNA polymerase II transcription subunit 4 Chain: s
Mediator of RNA polymerase II transcription subunit 7 Chain: t
Mediator of RNA polymerase II transcription subunit 9 Chain: u
Mediator of RNA polymerase II transcription subunit 10 Chain: v
Mediator of RNA polymerase II transcription subunit 19 Chain: w
Mediator of RNA polymerase II transcription subunit 21 Chain: x
Mediator of RNA polymerase II transcription subunit 31 Chain: y
Mediator of RNA polymerase II transcription subunit 15 Chain: z
super core promoter sense strand Chain: U
super core promoter antisense strand Chain: V

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.8Å
Relevant EMDB volumes: EMD-23255
Expression system: Escherichia coli