› 7lbw

X-ray diffraction
2.84Å resolution

Crystal structure of TFAM (mitochondrial transcription factor A) bridging two non-sequence specific DNA substrates

Released:
DOI: 10.2210/pdb7lbw/pdb
PDB entry 7lbw coloured by chain, front view.
PDB entry 7lbw coloured by chain, side view.
PDB entry 7lbw coloured by chain, top view.
Source organisms:
Primary publication:
A minimal motif for sequence recognition by mitochondrial transcription factor A (TFAM).
Choi WS, Garcia-Diaz M
OpenAccess logo Nucleic Acids Res 50 322-332 (2022)
PMID: 34928349

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121533 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription factor A, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 24.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00059 (Residues: 43-246; Coverage: 83%)
Gene names: TCF6, TCF6L2, TFAM
Sequence domains:
DNA (5'-D(P*TP*AP*GP*CP*CP*TP*TP*TP*CP*TP*AP*TP*TP*AP*GP*CP*TP*CP*TP*TP*AP*G)-3') Chains: C, E
Molecule details ›
Chains: C, E
Length: 22 nucleotides
Theoretical weight: 6.68 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*AP*GP*CP*CP*TP*TP*TP*CP*TP*AP*TP*TP*AP*GP*CP*TP*CP*TP*TP*AP*G)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 22 nucleotides
Theoretical weight: 6.82 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand EDO 6 x EDO
Ligand PEG 3 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 115Å b: 124.81Å c: 55.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.199 0.232
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 citation in other articles

DNA-protein cross-links between abasic DNA damage and mitochondrial transcription factor A (TFAM).
Xu et al. (2023)
4 more

1 mention without citation

A minimal motif for sequence recognition by mitochondrial transcription factor A (TFAM).
Choi et al. (2022)