› 7lep

Electron Microscopy
3.25Å resolution

The composite LBD-TMD structure combined from all hippocampal AMPAR subtypes at 3.25 Angstrom resolution

Released:
DOI: 10.2210/pdb7lep/pdb
PDB entry 7lep coloured by chain, front view.
PDB entry 7lep coloured by chain, side view.
PDB entry 7lep coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition.
Yu J, Rao P, Clark S, Mitra J, Ha T, Gouaux E
Nature 594 448-453 (2021)
PMID: 33981040
Related structures: EMD-23292

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-128651 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Mix of AMPAR subunits (GluA1, GluA3, and GluA4) Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 43.65 KDa
Source organism: Mus musculus
Glutamate receptor 2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 424 amino acids
Theoretical weight: 47.25 KDa
Source organism: Mus musculus
Mix of AMPAR subunits (GluA1, GluA3, and GluAX) Chain: C
Protein cornichon homolog 2 Chains: E, F
Voltage-dependent calcium channel gamma-8 subunit Chains: G, H

Ligands and Environments

7 bound ligands:
Ligand ZK1 4 x ZK1
Ligand POV 30 x POV
Ligand C14 1 x C14
Ligand D10 1 x D10
Ligand XVD 2 x XVD
Ligand OCT 2 x OCT
Ligand D12 2 x D12
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.25Å
Relevant EMDB volumes: EMD-23292

Quick links

Citations

9 review citations

Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology.
Klykov et al. (2022)
8 more

4 mentions without citation

Tetraspanins as Potential Modulators of Glutamatergic Synaptic Function.
Becic et al. (2021)
3 more