› 7lgn

X-ray diffraction
3.1Å resolution

Cyanophycin synthetase 1 from T. morbirosei

Released:
DOI: 10.2210/pdb7lgn/pdb
PDB entry 7lgn coloured by chain, front view.
PDB entry 7lgn coloured by chain, side view.
PDB entry 7lgn coloured by chain, top view.
Source organism: Tatumella morbirosei
Primary publication:
Structures and function of the amino acid polymerase cyanophycin synthetase.
Sharon I, Haque AS, Grogg M, Lahiri I, Seebach D, Leschziner AE, Hilvert D, Schmeing TM
Nat Chem Biol 17 1101-1110 (2021)
PMID: 34385683

Function and Biology Details

Reactions catalysed: 
ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp
ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100719 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyanophycin synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 909 amino acids
Theoretical weight: 98.65 KDa
Source organism: Tatumella morbirosei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A095T5Z8 (Residues: 1-900; Coverage: 100%)
Gene name: HA49_14190
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: I41
Unit cell:
a: 222.561Å b: 222.561Å c: 105.216Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.27 0.27 0.326
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Cyanophycin Modifications-Widening the Application Potential.
Kwiatos et al. (2021)
10 other articles