› 7lhx

X-ray diffraction
2.2Å resolution

Human U1A protein with F37M and F77M mutations for improved phasing

Released:
DOI: 10.2210/pdb7lhx/pdb
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PDB entry 7lhx coloured by chain, front view.
PDB entry 7lhx coloured by chain, side view.
PDB entry 7lhx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Affinity and Structural Analysis of the U1A RNA Recognition Motif with Engineered Methionines to Improve Experimental Phasing
Srivastava KY, Bonn-Breach RB, Chavali SS, Lippa GM, Jenkins JL, Wedekind JE
Crystals 11 273- (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140455 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
U1 small nuclear ribonucleoprotein A Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 1-98; Coverage: 35%)
Gene name: SNRPA
Sequence domains: RNA recognition motif

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand ACT 2 x ACT
Ligand BME 1 x BME
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BRUKER IMUS MICROFOCUS
Spacegroup: P321
Unit cell:
a: 64.377Å b: 64.377Å c: 46.828Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.213
Expression system: Escherichia coli BL21(DE3)

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