› 7lll

Electron Microscopy
3.7Å resolution

Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein

Released:
DOI: 10.2210/pdb7lll/pdb
PDB entry 7lll coloured by chain, front view.
PDB entry 7lll coloured by chain, side view.
PDB entry 7lll coloured by chain, top view.
Source organisms:
Primary publication:
Dynamics of GLP-1R peptide agonist engagement are correlated with kinetics of G protein activation.
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
OpenAccess logo Nat Commun 13 92 (2022)
PMID: 35013280
Related structures: EMD-23425

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-541230 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 1-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.41 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 58 amino acids
Theoretical weight: 6.38 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 5-62; Coverage: 82%)
Gene name: GNG2
Sequence domains: GGL domain
Nb35 Chain: N
Molecule details ›
Chain: N
Length: 128 amino acids
Theoretical weight: 13.89 KDa
Source organism: Lama glama
Expression system: Escherichia coli
Exendin-4 Chain: P
Molecule details ›
Chain: P
Length: 39 amino acids
Theoretical weight: 4.19 KDa
Source organism: Heloderma suspectum
Expression system: Escherichia coli
UniProt:
  • Canonical: P26349 (Residues: 48-86; Coverage: 61%)
Sequence domains: Peptide hormone
Glucagon-like peptide 1 receptor Chain: R
Molecule details ›
Chain: R
Length: 491 amino acids
Theoretical weight: 56.75 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P43220 (Residues: 24-463; Coverage: 100%)
Gene name: GLP1R
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.7Å
Relevant EMDB volumes: EMD-23425
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

4 review citations

Targeting VIP and PACAP Receptor Signaling: New Insights into Designing Drugs for the PACAP Subfamily of Receptors.
Lu et al. (2022)
3 more

6 mentions without citation

G protein-coupled receptors in neurodegenerative diseases and psychiatric disorders.
Wong et al. (2023)
5 more