› 7lmq

X-ray diffraction
1.91Å resolution

Structure of full-length human lambda-6A light chain JTO in complex with stabilizer 62 [4-methyl-3-(morpholinomethyl)-7-(1-phenylethoxy)-2H-chromen-2-one]

Released:
DOI: 10.2210/pdb7lmq/pdb
PDB entry 7lmq coloured by chain, front view.
PDB entry 7lmq coloured by chain, side view.
PDB entry 7lmq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Discovery of Potent Coumarin-Based Kinetic Stabilizers of Amyloidogenic Immunoglobulin Light Chains Using Structure-Based Design.
Yan NL, Santos-Martins D, Nair R, Chu A, Wilson IA, Johnson KA, Forli S, Morgan GJ, Petrassi HM, Kelly JW
J Med Chem 64 6273-6299 (2021)
PMID: 33939422

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-214383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
JTO light chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 23.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand 9ZW 1 x 9ZW
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 64.133Å b: 81.09Å c: 96.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.203
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Pharmacological stabilization of the native state of full-length immunoglobulin light chains to treat light chain amyloidosis.
Yan et al. (2023)
5 other articles

1 mention without citation

Discovery of Potent Coumarin-Based Kinetic Stabilizers of Amyloidogenic Immunoglobulin Light Chains Using Structure-Based Design.
Yan et al. (2021)