7lms

Electron Microscopy
3.5Å resolution

Structure of human SetD3 methyl-transferase in complex with 2A protease from Coxsackievirus B3

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
S-adenosyl-L-methionine + protein L-histidine = S-adenosyl-L-homocysteine + protein N(tau)-methyl-L-histidine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-136769 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protease 2A Chain: B
Molecule details ›
Chain: B
Length: 151 amino acids
Theoretical weight: 16.58 KDa
Source organism: Coxsackievirus B3 (strain Nancy)
Expression system: Escherichia coli
UniProt:
  • Canonical: P03313 (Residues: 855-1001; Coverage: 7%)
Sequence domains: Picornavirus core protein 2A
Actin-histidine N-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 596 amino acids
Theoretical weight: 67.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86TU7 (Residues: 2-594; Coverage: 100%)
Gene names: C14orf154, SETD3
Sequence domains:

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-23441
Expression system: Escherichia coli