› 7ln5

Electron Microscopy
3.09Å resolution

Cryo-EM structure of human p97 in complex with Npl4/Ufd1 and polyubiquitinated Ub-Eos (CHAPSO, Class 1, Close State)

Released:
DOI: 10.2210/pdb7ln5/pdb
PDB entry 7ln5 coloured by chain, front view.
PDB entry 7ln5 coloured by chain, side view.
PDB entry 7ln5 coloured by chain, top view.
Source organisms:
Primary publication:
Mechanistic insight into substrate processing and allosteric inhibition of human p97.
Pan M, Yu Y, Ai H, Zheng Q, Xie Y, Liu L, Zhao M
Nat Struct Mol Biol 28 614-625 (2021)
PMID: 34262183
Related structures: EMD-23449

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157191 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transitional endoplasmic reticulum ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 806 amino acids
Theoretical weight: 89.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55072 (Residues: 1-806; Coverage: 100%)
Gene names: HEL-220, HEL-S-70, VCP
Sequence domains:
polyubiquitinated Ub-Eos Chain: G
Molecule details ›
Chain: G
Length: 22 amino acids
Theoretical weight: 1.89 KDa
Source organism: Mus musculus
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
Ligand ADP 5 x ADP
Ligand ATP 7 x ATP
Ligand MG 7 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.09Å
Relevant EMDB volumes: EMD-23449
Expression system: Escherichia coli

Quick links

Citations

8 review citations

An expanded lexicon for the ubiquitin code.
Dikic et al. (2023)
7 more

2 mentions without citation

Insights into the Structure and Function of the Pex1/Pex6 AAA-ATPase in Peroxisome Homeostasis.
Judy et al. (2022)
1 more