› 7lnt

X-ray diffraction
2.35Å resolution

Ternary complex of the Isopentenyl Phosphate Kinase from Candidatus methanomethylophilus alvus bound to benzyl monophosphate and ATP

Released:
DOI: 10.2210/pdb7lnt/pdb
PDB entry 7lnt coloured by chain, front view.
PDB entry 7lnt coloured by chain, side view.
PDB entry 7lnt coloured by chain, top view.
Source organism: Candidatus Methanomethylophilus alvus
Primary publication:
Johnson BP, Kumar V, Scull EM, Thomas LM, Bourne CR, Singh S
ACS Chem Biol 17 85-102 (2022)
PMID: 34905349

Function and Biology Details

Reaction catalysed: 
ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-517054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.53 KDa
Source organism: Candidatus Methanomethylophilus alvus
Expression system: Escherichia coli
UniProt:
  • Canonical: M9SEQ1 (Residues: 1-259; Coverage: 100%)
Gene name: MMALV_14490
Sequence domains: Amino acid kinase family

Ligands and Environments

3 bound ligands:
Ligand GOL 2 x GOL
Ligand WZT 2 x WZT
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 40.698Å b: 74.662Å c: 170.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.234
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Molecular Basis for the Substrate Promiscuity of Isopentenyl Phosphate Kinase from Candidatus methanomethylophilus alvus.
Johnson et al. (2022)