Function and Biology Details
Reactions catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-S-ubiquitinyl-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domain:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Angiomotin; E3 ubiquitin-protein ligase NEDD4-like
Molecule details ›
Chain: A
Length: 68 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
Sequence domains: WW domain
Length: 68 amino acids
Theoretical weight: 7.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
- Canonical:
Q4VCS5 (Residues: 100-114; Coverage: 1%) - Canonical: Q96PU5 (Residues: 493-539; Coverage: 5%)
Sequence domains: WW domain
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
87%
Refinement method:
DGSA-distance geometry simulated annealing
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
