› 7lr2

X-ray diffraction
2.4Å resolution

Crystal structure of GH5_18 from Bifidobacterium longum subsp. longum ATCC 55813 in complex with GlcNAc

Released:
DOI: 10.2210/pdb7lr2/pdb
PDB entry 7lr2 coloured by chain, front view.
PDB entry 7lr2 coloured by chain, side view.
PDB entry 7lr2 coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. longum ATCC 55813
Primary publication:
N-Glycan Degradation Pathways in Gut- and Soil-Dwelling Actinobacteria Share Common Core Genes.
Higgins MA, Tegl G, MacDonald SS, Arnal G, Brumer H, Withers SG, Ryan KS
ACS Chem Biol 16 701-711 (2021)
PMID: 33764747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-245379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase BlGH5_18 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 446 amino acids
Theoretical weight: 49.62 KDa
Source organism: Bifidobacterium longum subsp. longum ATCC 55813
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

3 bound ligands:
Ligand EDO 21 x EDO
Ligand NAG 4 x NAG
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 105.559Å b: 143.486Å c: 155.893Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Carbohydrate-active enzymes (CAZymes) in the gut microbiome.
Wardman et al. (2022)
1 more