› 7lsa

X-ray diffraction
1.76Å resolution

Ruminococcus bromii Amy12 with maltoheptaose

Released:
DOI: 10.2210/pdb7lsa/pdb
PDB entry 7lsa coloured by chain, front view.
PDB entry 7lsa coloured by chain, side view.
PDB entry 7lsa coloured by chain, top view.
Source organism: Ruminococcus bromii
Primary publication:
Structure and substrate recognition by the Ruminococcus bromii amylosome pullulanases.
Cockburn DW, Kibler R, Brown HA, Duvall R, Moraïs S, Bayer E, Koropatkin NM
J Struct Biol 213 107765 (2021)
PMID: 34186214

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
hetero
Assembly name:
PDBe Complex ID:
PDB-CPX-496796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
pullulanase Chain: A
Molecule details ›
Chain: A
Length: 797 amino acids
Theoretical weight: 87.17 KDa
Source organism: Ruminococcus bromii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2N0UU23 (Residues: 1-797; Coverage: 75%)
Gene names: RBL236_00821, pulA_2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand GOL 8 x GOL
Ligand PEG 1 x PEG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 46.977Å b: 97.96Å c: 167.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.159 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Tutorial: Microbiome studies in drug metabolism.
Dodd et al. (2022)
1 more

1 mention without citation

Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome.
Cerqueira et al. (2022)