› 7lst

X-ray diffraction
2.05Å resolution

Ruminococcus bromii Amy12-D392A with 63-a-D-glucosyl-maltotriosyl-maltotriose

Released:
DOI: 10.2210/pdb7lst/pdb
PDB entry 7lst coloured by chain, front view.
PDB entry 7lst coloured by chain, side view.
PDB entry 7lst coloured by chain, top view.
Source organism: Ruminococcus bromii
Primary publication:
Structure and substrate recognition by the Ruminococcus bromii amylosome pullulanases.
Cockburn DW, Kibler R, Brown HA, Duvall R, Moraïs S, Bayer E, Koropatkin NM
J Struct Biol 213 107765 (2021)
PMID: 34186214

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-496796 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
pullulanase Chain: A
Molecule details ›
Chain: A
Length: 800 amino acids
Theoretical weight: 87.46 KDa
Source organism: Ruminococcus bromii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2N0UU23 (Residues: 1-800; Coverage: 75%)
Gene names: RBL236_00821, pulA_2
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
4 bound ligands:
Ligand GOL 11 x GOL
Ligand ACT 8 x ACT
Ligand CA 1 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 46.981Å b: 99.107Å c: 167.236Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 citation in other articles

Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome.
Cerqueira et al. (2022)
2 more