7lte

X-ray diffraction
2Å resolution

Structure of the alpha-N-methyltransferase (SonM) and RiPP precursor (SonA) heteromeric complex (with SAH)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-184393 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tetrapyrrole methylase domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 263 amino acids
Theoretical weight: 29.07 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EGW3 (Residues: 1-263; Coverage: 100%)
Gene name: SO_1478
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
LigA domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EGW2 (Residues: 1-71; Coverage: 100%)
Gene name: SO_1479

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 52.46Å b: 108.66Å c: 59.09Å
α: 90° β: 94.1° γ: 90°
R-values:
R R work R free
0.214 0.212 0.244
Expression system: Escherichia coli