7lts

X-ray diffraction
2.32Å resolution

Structure of the alpha-N-methyltransferase (SonM mutant R67A) and RiPP precursor (SonA) heteromeric complex (with SAH)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-184393 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tetrapyrrole methylase domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 263 amino acids
Theoretical weight: 28.98 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EGW3 (Residues: 1-263; Coverage: 100%)
Gene name: SO_1478
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
LigA domain-containing protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 73 amino acids
Theoretical weight: 8.1 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8EGW2 (Residues: 1-71; Coverage: 100%)
Gene name: SO_1479

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P41212
Unit cell:
a: 80.95Å b: 80.95Å c: 236.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.198 0.248
Expression system: Escherichia coli