› 7lyf

X-ray diffraction
3.4Å resolution

Dengue virus RNA promoter stem-loop A fused with tRNA-scaffold

Released:
DOI: 10.2210/pdb7lyf/pdb
PDB entry 7lyf coloured by chain, front view.
PDB entry 7lyf coloured by chain, side view.
PDB entry 7lyf coloured by chain, top view.
Source organism: Dengue virus 2
Primary publication:
Structures of flavivirus RNA promoters suggest two binding modes with NS5 polymerase.
Lee E, Bujalowski PJ, Teramoto T, Gottipati K, Scott SD, Padmanabhan R, Choi KH
OpenAccess logo Nat Commun 12 2530 (2021)
PMID: 33953197

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-250399 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA promoter stem-loop A fused with tRNA Chain: A
Molecule details ›
Chain: A
Length: 139 nucleotides
Theoretical weight: 44.87 KDa
Source organism: Dengue virus 2
Expression system: Escherichia coli
Sequence domains:
  • tRNA
  • Dengue virus SLA

    • Ligands and Environments

    1 bound ligand:
    Ligand MG 1 x MG
    No modified residues

    Experiments and Validation Details

    Entry percentile scores
    X-ray source: APS BEAMLINE 21-ID-F
    Spacegroup: I222
    Unit cell:
    a: 30.924Å b: 137.558Å c: 336.877Å
    α: 90° β: 90° γ: 90°
    R-values:
    R R work R free
    0.23 0.226 0.268
    Expression system: Escherichia coli

    Quick links

    Citations

    4 review citations

    Dengue Virus Infection: A Tale of Viral Exploitations and Host Responses.
    Nanaware et al. (2021)
    3 more

    1 mention without citation

    Structural basis for cloverleaf RNA-initiated viral genome replication.
    Gottipati et al. (2023)