› 7lz3

X-ray diffraction
2.18Å resolution

Computational design of constitutively active cGAS

Released:
DOI: 10.2210/pdb7lz3/pdb
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PDB entry 7lz3 coloured by chain, front view.
PDB entry 7lz3 coloured by chain, side view.
PDB entry 7lz3 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Computational design of constitutively active cGAS.
Dowling QM, Volkman HE, Gray EE, Ovchinnikov S, Cambier S, Bera AK, Sankaran B, Johnson MR, Bick MJ, Kang A, Stetson DB, King NP
Nat Struct Mol Biol (2023)
PMID: 36593311

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic GMP-AMP synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 362 amino acids
Theoretical weight: 42.41 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 47.238Å b: 106.363Å c: 75.667Å
α: 90° β: 91.132° γ: 90°
R-values:
R R work R free
0.214 0.211 0.265
Expression system: Escherichia coli K-12

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Citations

2 review citations

How Does cGAS Avoid Sensing Self-DNA under Normal Physiological Conditions?
Zheng et al. (2023)
1 more

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