› 7m3p

X-ray diffraction
2Å resolution

Xrcc4-Spc110p(164-207) fusion

Released:
DOI: 10.2210/pdb7m3p/pdb
Model geometry
Fit model/data
PDB entry 7m3p coloured by chain, front view.
PDB entry 7m3p coloured by chain, side view.
PDB entry 7m3p coloured by chain, top view.
Source organisms:
Primary publication:
CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
OpenAccess logo Elife 10 (2021)
PMID: 33949948

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152290 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair protein XRCC4; Spindle pole body component 110 Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.73 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13426 (Residues: 2-132; Coverage: 39%)
  • Canonical: P32380 (Residues: 164-207; Coverage: 5%)
Gene names: D9476.3, NUF1, SPC110, XCM1, XRCC4, YDR356W
Sequence domains: XRCC4 N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P1
Unit cell:
a: 38.131Å b: 52.168Å c: 55.916Å
α: 100.804° β: 94.148° γ: 112.831°
R-values:
R R work R free
0.206 0.204 0.247
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Molecular insight into how γ-TuRC makes microtubules.
Thawani et al. (2021)
3 more

1 mention without citation

CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot et al. (2021)