› 7m5c

X-ray diffraction
3.06Å resolution

Crystal Structure of human BAK in complex with WT BAK BH3 peptide

Released:
DOI: 10.2210/pdb7m5c/pdb
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PDB entry 7m5c coloured by chain, front view.
PDB entry 7m5c coloured by chain, side view.
PDB entry 7m5c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of BAK activation in mitochondrial apoptosis initiation.
Singh G, Guibao CD, Seetharaman J, Aggarwal A, Grace CR, McNamara DE, Vaithiyalingam S, Waddell MB, Moldoveanu T
OpenAccess logo Nat Commun 13 250 (2022)
PMID: 35017502

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-278778 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2 homologous antagonist/killer Chains: A, C, E, G, I, K, M, O, Q, S
Molecule details ›
Chains: A, C, E, G, I, K, M, O, Q, S
Length: 166 amino acids
Theoretical weight: 18.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16611 (Residues: 21-186; Coverage: 79%)
  • Best match: Q16611-2 (Residues: 21-116)
Gene names: BAK, BAK1, BCL2L7, CDN1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Bcl-2 homologous antagonist/killer Chains: B, D, F, H, J, L, N, P, R, T
Molecule details ›
Chains: B, D, F, H, J, L, N, P, R, T
Length: 25 amino acids
Theoretical weight: 2.67 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16611 (Residues: 68-89; Coverage: 10%)
Gene names: BAK, BAK1, BCL2L7, CDN1

Ligands and Environments

2 bound ligands:
Ligand CU 9 x CU
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS-II BEAMLINE 17-ID-2
Spacegroup: P21
Unit cell:
a: 89.385Å b: 132.215Å c: 89.379Å
α: 90° β: 119.2° γ: 90°
R-values:
R R work R free
0.216 0.213 0.249
Expression systems:
  • Escherichia coli
  • Not provided

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Citations

5 review citations

Mechanisms of BCL-2 family proteins in mitochondrial apoptosis.
Czabotar et al. (2023)
4 more

2 mentions without citation

Peptides from human BNIP5 and PXT1 and non-native binders of pro-apoptotic BAK can directly activate or inhibit BAK-mediated membrane permeabilization.
Aguilar et al. (2023)
1 more

PDB-REDO

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