› 7m6j

Electron Microscopy
3.6Å resolution

Human Septin Hexameric Complex SEPT2G/SEPT6/SEPT7 by Single Particle Cryo-EM

Released:
DOI: 10.2210/pdb7m6j/pdb
PDB entry 7m6j coloured by chain, front view.
PDB entry 7m6j coloured by chain, side view.
PDB entry 7m6j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An atomic model for the human septin hexamer by cryo-EM.
Mendonça DC, Guimarães SL, Pereira HD, Pinto AA, de Farias MA, de Godoy AS, Araujo APU, van Heel M, Portugal RV, Garratt RC
J Mol Biol 433 167096 (2021)
PMID: 34116125
Related structures: EMD-23698

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171931 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Septin-2 Chains: A, F
Molecule details ›
Chains: A, F
Length: 275 amino acids
Theoretical weight: 31.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15019 (Residues: 35-308; Coverage: 76%)
Gene names: DIFF6, KIAA0158, NEDD5, SEPT2, SEPTIN2
Sequence domains: Septin
Septin-6 Chains: B, E
Molecule details ›
Chains: B, E
Length: 427 amino acids
Theoretical weight: 48.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14141 (Residues: 1-427; Coverage: 98%)
Gene names: KIAA0128, SEP2, SEPT6, SEPTIN6
Sequence domains: Septin
Septin-7 Chains: C, D
Molecule details ›
Chains: C, D
Length: 432 amino acids
Theoretical weight: 50.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16181 (Residues: 20-437; Coverage: 96%)
Gene names: CDC10, SEPT7, SEPTIN7
Sequence domains: Septin

Ligands and Environments

3 bound ligands:
Ligand GDP 4 x GDP
Ligand GTP 2 x GTP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-23698
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The emerging role of septins in fungal pathogenesis.
Eisermann et al. (2023)
1 more

6 mentions without citation

The Structural Biology of Septins and Their Filaments: An Update.
Cavini et al. (2021)
5 more