› 7m6k

X-ray diffraction
1.69Å resolution

Crystal structure of the ARM domain from Drosophila SARM1 in complex with VMN

Released:
DOI: 10.2210/pdb7m6k/pdb
PDB entry 7m6k coloured by chain, front view.
PDB entry 7m6k coloured by chain, side view.
PDB entry 7m6k coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Neurotoxin-mediated potent activation of the axon degeneration regulator SARM1.
Loreto A, Angeletti C, Gu W, Osborne A, Nieuwenhuis B, Gilley J, Merlini E, Arthur-Farraj P, Amici A, Luo Z, Hartley-Tassell L, Ve T, Desrochers LM, Wang Q, Kobe B, Orsomando G, Coleman MP
OpenAccess logo Elife 10 (2021)
PMID: 34870595

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(+) hydrolase sarm1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 34.4 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6IDD9 (Residues: 369-678; Coverage: 23%)
Gene names: CG43119, Ect4, Sarm

Ligands and Environments

2 bound ligands:
Ligand YRJ 2 x YRJ
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P1
Unit cell:
a: 39.065Å b: 51.01Å c: 76.56Å
α: 103.492° β: 101.784° γ: 96.071°
R-values:
R R work R free
0.191 0.19 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Multifaceted roles of SARM1 in axon degeneration and signaling.
Waller et al. (2022)
3 more