› 7m9b

Electron Microscopy
3.8Å resolution

ADP-AlF3 bound TnsC structure in closed form

Released:
DOI: 10.2210/pdb7m9b/pdb
PDB entry 7m9b coloured by chain, front view.
PDB entry 7m9b coloured by chain, side view.
PDB entry 7m9b coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for target site selection in RNA-guided DNA transposition systems.
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science 373 768-774 (2021)
PMID: 34385391
Related structures: EMD-23722

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-121618 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TnsC Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 276 amino acids
Theoretical weight: 31.44 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli BL21
DNA (27-MER) Chain: M
Molecule details ›
Chain: M
Length: 27 nucleotides
Theoretical weight: 8.41 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (27-MER) Chain: N
Molecule details ›
Chain: N
Length: 27 nucleotides
Theoretical weight: 8.17 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ADP 10 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-23722
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

CRISPR-based genome editing through the lens of DNA repair.
Nambiar et al. (2022)
8 more

1 mention without citation

Structural basis for target site selection in RNA-guided DNA transposition systems.
Park et al. (2021)