› 7mbj

X-ray diffraction
1.26Å resolution

Crystal structure of cGMP dependent protein kinase I alpha (PKG I alpha)CNB-A domain with R177Q mutation

Released:
DOI: 10.2210/pdb7mbj/pdb
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PDB entry 7mbj coloured by chain, front view.
PDB entry 7mbj coloured by chain, side view.
PDB entry 7mbj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An auto-inhibited state of protein kinase G and implications for selective activation.
Sharma R, Kim JJ, Qin L, Henning P, Akimoto M, VanSchouwen B, Kaur G, Sankaran B, MacKenzie KR, Melacini G, Casteel DE, Herberg FW, Kim CW
OpenAccess logo Elife 11 (2022)
PMID: 35929723

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171844 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cGMP-dependent protein kinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13976 (Residues: 79-212; Coverage: 20%)
Gene names: PRKG1, PRKG1B, PRKGR1A, PRKGR1B
Sequence domains: Cyclic nucleotide-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P1
Unit cell:
a: 39.198Å b: 41.855Å c: 48.312Å
α: 94.739° β: 113.664° γ: 114.027°
R-values:
R R work R free
0.178 0.178 0.197
Expression system: Escherichia coli

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Citations

1 review citation

Dietary Beetroot Juice - Effects in Patients with COPD: A Review.
Chen et al. (2024)
1 other article

2 mentions without citation

Fluorinated derivatives of pyridine-2,4-dicarboxylate are potent inhibitors of human 2-oxoglutarate dependent oxygenases.
Brewitz et al. (2021)
1 more

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