› 7mbu

Electron Microscopy

Cryo-EM structure of zebrafish TRPM5 E337A mutant in the presence of 5 mM calcium (high calcium occupancy in the transmembrane domain)

Released:
DOI: 10.2210/pdb7mbu/pdb
PDB entry 7mbu coloured by chain, front view.
PDB entry 7mbu coloured by chain, side view.
PDB entry 7mbu coloured by chain, top view.
Source organism: Danio rerio
Primary publication:
Structures of the TRPM5 channel elucidate mechanisms of activation and inhibition.
Ruan Z, Haley E, Orozco IJ, Sabat M, Myers R, Roth R, Du J, Lü W
Nat Struct Mol Biol 28 604-613 (2021)
PMID: 34168372
Related structures: EMD-23747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transient receptor potential cation channel subfamily M member 5 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1165 amino acids
Theoretical weight: 132.74 KDa
Source organism: Danio rerio
Expression system: Homo sapiens
UniProt:
  • Canonical: S5UH55 (Residues: 1-1165; Coverage: 100%)
Gene name: trpm5
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 4 x NAG
Ligand CA 4 x CA
Ligand YUY 4 x YUY
Ligand YUV 4 x YUV
No modified residues

Experiments and Validation Details

Entry percentile scores
Relevant EMDB volumes: EMD-23747
Expression system: Homo sapiens

Quick links

Citations

10 review citations

TRP (transient receptor potential) ion channel family: structures, biological functions and therapeutic interventions for diseases.
Zhang et al. (2023)
9 more

2 mentions without citation

Structures of the TRPM5 channel elucidate mechanisms of activation and inhibition.
Ruan et al. (2021)
1 more