› 7mcm

X-ray diffraction
2.05Å resolution

Crystal Structure of Enoyl-CoA hydratase from Mycolicibacterium smegmatis

Released:
DOI: 10.2210/pdb7mcm/pdb
PDB entry 7mcm coloured by chain, front view.
PDB entry 7mcm coloured by chain, side view.
PDB entry 7mcm coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-CoA hydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 260 amino acids
Theoretical weight: 27.52 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0QXQ8 (Residues: 2-257; Coverage: 100%)
Gene name: MSMEG_3390
Sequence domains: Enoyl-CoA hydratase/isomerase

Ligands and Environments

1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P63
Unit cell:
a: 104.13Å b: 104.13Å c: 83.75Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.199
Expression system: Escherichia coli BL21(DE3)

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